Reporter

Part:BBa_K3026001:Experience

Designed by: Francois D Rouleau   Group: iGEM19_ULaval   (2019-09-08)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3026001

ToeHold_RNA_AmpR

ToeHold switch targeting mRNA sequence of pBluescript ampicillin resistance cassette. This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the mRNA sequence of the ampicillin resistance cassette. When this ToeHold is in presence of this mRNA sequence, the sfGFP will be produced. This system allows for detection of DNA fragments or plasmids expressing this cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


User Reviews

UNIQd16d96d68416ab74-partinfo-00000002-QINU UNIQd16d96d68416ab74-partinfo-00000003-QINU